Chromatogram

A submenu of commands for working with chromatograms.

	Global Peak Width	The Global Peak Width command sets the minimum peak width to be integrated across the entire chromatogram. It should correspond to the narrowest peak that still has to be detected; peaks narrower than this value will not be integrated. The value can be defined by invoking the command or clicking the icon, which applies the parameter for the whole chromatogram based on the interactively selected interval. Alternatively, the Global Peak Width can also be set manually in the first row of the Integration Table found on the Chromatogram - Integration tab. The Global Peak Width also serves as the basis for calculating the Global Filter – Bunching parameter (see below).
	Global Threshold	The Global Threshold command sets the noise threshold for the entire chromatogram. The Threshold defines the sensitivity of the integration algorithm to distinguish peaks from noise. Peaks to be detected must have a height at least twice the threshold value. The value can be calculated by invoking the command or clicking the icon, which determines the parameter for the whole chromatogram based on the interactively selected interval. Alternatively, the Global Threshold can also be set manually in the second row of the Integration Table found on the Chromatogram - Integration tab.
	Global Filter - Bunching	The Global Filter - Bunching averages a defined number of adjacent data points into one, which reduces noise and the number of recorded data points while keeping sufficient resolution for peak evaluation. The value can be calculated by invoking the command or clicking the icon, which determines the parameter for the whole chromatogram based on the set Global Peak Width value and the data acquisition frequency, ensuring that a peak defined by the Global Peak Width is represented by approximately 30 data points. Alternatively, the Global Filter - Bunching can also be set manually in the third row of the Integration Table found on the Chromatogram - Integration tab.
	Global Baseline Slope	The Global Baseline Slope command is available only in the Wave integration algorithm. It sets the expected signal curve slope for the entire chromatogram and is used to determine the positions of peak starts and ends. The value can be calculated by invoking the command or clicking the icon, which determines the parameter based on an interactively selected position in the graph. Alternatively, the Global Baseline Slope can also be set manually in the fourth row of the Integration Table found on the Chromatogram - Integration tab. If the slope is not defined (value set to 0), peak start and end positions are derived from the Threshold parameter (8 * Threshold), which is the same principle used in the Legacy integration algorithm.
	Baseline	Commands for baseline correction. Detailed description of Baseline parameters can be found in the chapter "Baseline".
	Peak	Commands for operations with peaks. Detailed description of operations with peaks can be found in the chapter "Peak".
	Integration	Commands for adjustment of integration parameters. Detailed description of Integration options can be found in the chapter "Integration".
	Separation	Commands for adjustment of separation parameters. Detailed description of Separation parameters can be found in the chapter "Separation".
	Noise & Drift	Commands for evaluation of noise and drift. Detailed description of Noise & Drift operations can be found in the chapter "Noise & Drift".
	Overlay	Commands for simultaneous work with several chromatograms. Detailed description of Overlay and related options can be found in the chapter "Overlay & Other".
	Chromatogram list	Contains a chronological list of all currently displayed chromatograms. The active chromatogram is denoted by a point before its name. To set a chromatogram to active status, click on its name. Chromatograms can be displayed using the Chromatograms… command.
	Chromatograms…	Displays the Chromatogram List dialog with a list of all chromatograms/signals that are opened in the Chromatogram window.
	Measure Distance	Shows the distance to the relative point in the graph. After invoking the command or clicking on the Measure Distance icon the cursor changes to and will prevent you from leaving the Graph pane. Click and drag to see the distance from the location you clicked on and the current cursor position. This distance is shown just out of the upper right corner of the Graph pane. Normally, absolute position of the cursor in the graph is shown.
	Show Slope/Level	Shows the Slope and Level values of the nearest acquired data point (of the signal line) at the current cursor position. After invoking the command or clicking on the Show Slope/Level icon the cursor changes to a balloon tooltip, which prevents you from leaving the Graph pane. The balloon tooltip shows values of Time, FC Slope, Level and IA Slope related to the current cursor position. The difference between the values of FC Slope and IA Slope is caused by the signal rate (FC Slope does not take frequency into account) and by the calculation (FC Slope is calculated as a non-symmetrical derivative, because at the time of measurement further points are not known yet). Values displayed by the Show Slope/Level function are influenced by the Global Filter - Bunching setting in the Integration Table. If you want to use Show Slope/Level values to fill the Fraction table for fraction collectors, the Global Filter - Bunching value in the Integration Table must match theFilter setting in the Fraction Table.
	Create Label	Adds labels and lines. Detailed description on how to create labels can be found in the chapter "Create Label".
	Remove Label(s)	Deletes labels and lines. Selected Deletes the selected label or line. From Workplace Deletes all labels and lines attached to the workplace. From Active Signal Deletes all labels and lines attached to the active signal. Remove All Deletes all labels and lines regardless of whether they are attached to the workplace or to the active chromatogram. Labels of other opened but inactive chromatograms will be retained.
	Merge…	Creates multi-detector chromatograms. The command is commonly used when a multi-detector analysis was measured on one of the CSW stations which do not allow for the creation of multi-detector chromatograms. The procedure is following: Open the chromatogram to which you would like to add further signals. Prompt the Merge command and the Open Chromatogram dialog will open where you can select a chromatogram. Select the required chromatogram and click the OK button. Repeat the procedure to add more signals. It is possible to create a chromatogram containing a maximum of 32 signals. Only the whole chromatograms will be merged, regardless of the number of signals selected in Open Chromatogram dialog. Chromatograms containing PDA data cannot be merged.
	Set Signal Names…	Edits names of signals.