Chromatogram

A submenu of commands for working with chromatograms.

Global Peak Width Use of this command or Global Peak Width icon calculates the Peak Width parameter for the whole chromatogram from interactively selected interval. The Global Peak Width can also be set manually in the first row of the Integration Table found on the Chromatogram - Integration tab).

Detailed description of the Peak Width parameter can be found in the chapter "Integration".
Global Threshold Use of this command or Global Threshold icon calculates the Threshold parameter for the whole chromatogram from interactively selected interval. The Global Threshold can also be set manually in the second row of the Integration Table found on the Chromatogram - Integration tab).

Detailed description of the Threshold parameter is in the chapter "Integration".
Global Bunching Use of this command or Global Bunching icon calculates the Global Bunching parameter for the whole chromatogram from already entered Global Peak Width value and the frequency of the data acquisition. The calculation sets the Global Bunching parameter so that the peak (as defined in the Global Peak Width) has approximately 30 datapoints. The Global Bunching can also be set manually in the third row of the Integration Table found on the Chromatogram - Integration tab.
Global Baseline Slope Use of this command or Global Baseline Slope icon calculates the Global Baseline Slope parameter for the whole chromatogram from interactively selected graph position. Because of the noise present in the signal the position of peak starts and ends will be just approximate. The Global Baseline Slope can also be set manually in the fourth row of the Integration Table found on the Chromatogram - Integration tab. The command is only available if Wave Integration Algorithm is used.
  Baseline Commands for baseline correction.

Detailed description of Baseline parameters can be found in the chapter "Baseline".
  Peak Commands for operations with peaks.

Detailed description of operations with peaks can be found in the chapter "Peak".
  Integration Commands for adjustment of integration parameters.

Detailed description of Integration options can be found in the chapter "Integration".
  Separation Commands for adjustment of separation parameters.

Detailed description of Separation parameters can be found in the chapter "Separation".
  Noise & Drift Commands for evaluation of noise and drift.

Detailed description of Noise & Drift operations can be found in the chapter "Noise & Drift".
Overlay Commands for simultaneous work with several chromatograms.

Detailed description of Overlay and related options can be found in the chapter "Overlay & Other".
  Chromatogram list Contains a chronological list of all currently displayed chromatograms. The active chromatogram is denoted by a point before its name. To set a chromatogram to active status, click on its name. Chromatograms can be displayed using the Chromatograms… command.
  Chromatograms… Displays the Chromatogram List dialog with a list of all chromatograms/signals that are opened in the Chromatogram window.
Measure Distance Shows the distance to the relative point in the graph. After invoking the command or clicking on the Measure Distance icon the cursor changes to and will prevent you from leaving the Graph pane. Click and drag to see the distance from the location you clicked on and the current cursor position. This distance is shown just out of the upper right corner of the Graph pane. Normally, absolute position of the cursor in the graph is shown.
Show Slope/Level Shows Slope and Level values of the nearest acquired data point (of the signal line) to a current cursor position. After invoking the command or clicking on the Show Slope/Level icon the cursor changes to a balloon tooltip will prevent you from leaving the Graph pane. The balloon tooltip shows values of Time, FC Slope, Level and IA Slope related to the current cursor position. The difference between the values of FC Slope and IA Slope is caused by the signal rate (FC Slope does not take frequency into account) and the way of calculation (FC Slope is calculated as a non-symetrical derivative because at the time of measurement the further points are not known yet).
 

Note:

Note that values displayed by Show Slope/Level function are influenced by the set Global Bunching value in the Integration Table. If you need to display Show Slope/Level values in order to fill them in Fraction table for the fraction collectors, is necessary to have the same values set for the Global Bunching in the Integration Table and Filter in the Fraction Table.
  Create Label Adds labels and lines.

Detailed description on how to create labels can be found in the chapter "Create Label".
  Remove Label(s) Deletes labels and lines.

Selected

Deletes the selected label or line.

From Workplace

Deletes all labels and lines attached to the workplace.

From Active Signal

Deletes all labels and lines attached to the active signal.

Remove All

Deletes all labels and lines regardless of whether they are attached to the workplace or to the active chromatogram. Labels of other opened but inactive chromatograms will be retained.
  Merge… Creates multi-detector chromatograms. The command is commonly used when a multi-detector analysis was measured on one of the CSW stations which do not allow for the creation of multi-detector chromatograms. The procedure is following:
  1. Open the chromatogram to which you would like to add further signals.
  2. Prompt the Merge command and the Open Chromatogram dialog will open where you can select a chromatogram.
  3. Select the required chromatogram and click the OK button.
  4. Repeat the procedure to add more signals.

It is possible to create a chromatogram containing a maximum of 32 signals. Only the whole chromatograms will be merged, regardless of the number of signals selected in Open Chromatogram dialog. Chromatograms containing PDA data cannot be merged.
  Set Signal Names… Edits names of signals.