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GUIDES & DOCUMENTATION
- Adjusting display of chromatogram You can customize the appearance of the chromatogram graph using the Graph Properties dialog available in the Chromatogram window to suit your specific needs. Below…
- Panes can be adjusted in the Chromatogram and Calibration windows. It applies to horizontal as well as vertical splitters. Double click on the area next to the border (the cursor changes to ) to…
- Sets other parameters of the method, most notably the Subtraction and Auxiliary Signals. When invoked by the menu command, the Method Setup - Advanced dialog will open. The tab is common to all…
- The AIA file format has relatively strictly designed form, but not the content. Thus, an AIA file may contain various custom items. The dialog for importing files in AIA format is designed as an…
- All Signals Result Table Invoking this command switches the lower part of the Chromatogram window to the All Signals Results tab, displaying the All Signals Result Table in the left half. When using…
- This is a submenu containing commands for measurement control: Icon Command Description Single... Opens the Single Analysis dialog, allowing users to prepare and perform a single…
- Submenu with commands for controlling the running of the analysis. Send Method It sends the method to all connected control modules/hardware. This button is not available during data…
- Chapters describing some other aspects of using Clarity and help.
- Retention Index Also known as the Kováts retention index. For detected compounds, the Retention Index (RI) is calculated according to the following formula: where tr,j is the reduced retention…
- Match Factor The Match Factor is calculated using the normalized spectral data on the basis of the Match Criteria selected in the Chromatogram - Measurement Conditions - PDA Method tab. In case the…
- Mathematical formulas Parameter EP/USP/JP USP until 12/2022 JP until 3/2006 Plate Numberi/ Efficiencyi Assymetryi Symmetryi/ Tailingi Capacityi Eff/li…
- Search performed in the MS Search program may follow dozens of different settings. Clarity software does not directly offer all of these settings, but some may be forced to Clarity searches using…
- Search performed in the MS Search program may follow dozens of different settings. Clarity software does not directly offer all of these settings, but some may be forced to Clarity searches using…
- If the calibration file is not assigned to the method, the measured chromatogram will not have it linked either. To link a calibration file to a chromatogram do as follows: Switch to the GPC…
- Archive Archives or erases selected files or entire projects in the Clarity station. The command opens the Backup - Create Archive dialog. Files from the current project can be backed up to any…
- Clarity allows you to archive your Projects (methods, sequences, measured chromatograms) and also the Common folder in which the print styles are stored.
- It is strongly recommended to archive the whole project folder after being shelved, but it is also possible to archive specific files only. An archive can be made by simply copying the files or by…
- It is strongly recommended to archive the whole project folder after being shelved but it is also possible to archive specific files only. An archive can be made by simply copying the files or by…
- All data must be archived for the period specified by the relevant regulatory authorities. This requirement is mandated by 21 CFR Part 11 and GLP. Note: The FDA's version of the GLP specifies…
- Selection of autosampler sampling program. When prompted, the AS tab of the Method Setup dialog will be displayed. The command will only be functional if the respective control module has been…
- AS + GC set - Active Sequence In GC systems, the sample cycle is typically controlled by the GC, as the cool-down time of different systems varies due to the generally used temperature gradient. The…
- The autosampler used in the Passive Sequence does not need the OUTnR digital output cable to be connected. All timings are controlled by the chromatograph and autosampler. Clarity performs only one…
- AS + LC set - Active Sequence In LC systems, the autosampler typically governs the timings. The eventual pump gradient and detector programs are set independently. Any autosampler that is used in…
- When using the optional AS Control (p/n A26) module, all communication is performed through a separate data cable (usually a serial cable connected to a COM port). Caution: Refer to the…
- When using optional AS Control module in combination with digital acquisition detectors (e.g., the Agilent 6890 module), the connection should be as follows. All communication with Clarity is…
- Assigning digital input and output to start acquisition There are basically three means when Clarity decides to start the acquisition: User presses Run button in Single Analysis (user probably did…
- Oven Linear carrier flow (He) 2.4 ml/min Initial temperature 35 °C First hold temperature 5 °C First hold time 10 min First program rate 5 °C/min Second temperature ≈ 49 Second hold time ≈ 55…
- Oven Initial temperature 5 °C Initial time 10 min First program rate 5 °C/min First hold temperature 50 °C First hold time 0 min Second program rate 1.5 °C/min Final temperature 200 °C Final hold…
- Oven Initial temperature 35 °C Initial time 15 min First program rate 1 °C/min First hold temperature 60 °C First hold time 20 min Second program rate 2 °C/min Final temperature 200 °C Final hold…
- Audit & Signatures Tabs: Page Setup - Lab. Header - Report Header - Method - Calibration - Chromatogram - Results - Sequence - SST - MS - Audit & Signatures - PDA - NGA - Lab. Footer…
- Audit Trail window is used for logging of individual operations of the station. By allowing backward control of all performed operations, including information about who and when it was performed,…
- The most typical autosampler connections are described in the User Guide in the chapter "Connecting Autosamplers (AS)". The start synchronization configuration through Ext. Start Dig. Input and…
- Aux. Signals Sets the auxiliary signals storing and possible display. The tab is present only when the Auxiliary signals are available, and is common to all signals while performing multidetector…
- Available Control Modules The Available Control Modules dialog allows to add the particular control module driver into the System Configuration dialog. Try to search the driver by its name using the…
- Switching Windows user profiles or entering sleep mode while Clarity is running can disrupt communication with the hardware key or connected instruments. Active Clarity station (with an Instrument…
- Axes Appearance tab Tabs: General - Graph - Axes Appearance - Signals & Curves - Gradient & Auxiliary Signals - Directories Sets appearance options for the graph axes. User Options - Axes…
- Axes Ranges The Axes Ranges dialog, serving for setting the axes ranges both during the acquisition run and outside of it, can be invoked using the Set Axes Ranges… command from the View menu of the…
- Baseline Processes the baseline. Individual commands can also be invoked with the icons in the Baseline toolbar. Baseline Toolbar All operations involving the use of peaks are performed on an…
- Basic principles and terms Elemental Analysis (EA) is an analytical technique used for determination of elemental composition of various samples. The contents of common elements (C, H, N, O, S) are…
- Multidimensional gas chromatography (GCxGC) is an automated analytical separation method based on separation of analytes on two different analytical columns with different separation properties.…
- Gel Permeation Chromatography (GPC) or Size Exclusion Chromatography (SEC) is a specialized chromatography technique for determination of molecular weight distribution in polymers. Sample of polymer…
- Basic principles and terms Mass Spectrometry (MS) is an analytical detection method that determines the structure of compounds from the ions to which it is fragmented. m/z means Mass to Charge…
- Basic principles and terms Mass Spectrometry (MS) is an analytical detection method that determines the structure of compounds from the ions to which the compound is fragmented. MS Spectrometer is…
- PDA View: PDA views are the different ways of displaying the acquired PDA data in the customizable PDA Chromatogram window. Up to four views can be displayed at one time; any combination from the…
- Batch Batch processes single chromatograms, automatically and simultaneously executes selected actions with numerous chromatograms or reprocesses whole sequences. When invoked, the Batch dialog…
- This chapter focuses on processing more chromatograms using Batch dialog. There are 3 main ways of workflow Reprocessing whole sequence, Reprocessing by Method, and Post-run Options. All will be…
- Best Practices for Using Clarity Effectively To ensure Clarity operates smoothly and delivers accurate results, it is important to follow certain practices when working with the software and your…
- This method first requires the calibration of the column with a series of Narrow Standards and then a calibration with a Broad Standard with known Mn and Mw. It relies on a principle that Narrow…
- Browse through chromatograms from the last folder opened using Open Chromatogram command or browses between selected chromatograms if you selected the "Browse selected chromatograms" option when…
- This is a standard procedure used across various chromatography applications - a known amount of a sample is dissolved in a known volume of a solvent and the goal is to determine the percentage…