I don't have an autosampler. Can I use the sequence table to start acquisition in such case?
Yes, you can. There are two possible intentions of using the sequence table for starting the acquisition:
1. Using sequence for manual injections
You could define parameters for a series of injections in advance in a sequence table and then inject them one by one manually. In such case start must be external (individual runs in sequence cannot be started from Clarity).
2. Using sequence for automatic injections without an autosampler
In such case sequence must be in the active mode (set through Sequence Options dialog). Sequence sends a command to the sampler (over serial line or by digital output) and waits for the start signal from the detector (again over the communication line or digital input). Without autosampler it is possible to start the acquisition by using the digital input and output specified in Ext. Start Dig. Input and Ready Dig. Output section of the System Configuration dialog.
How to set digital input and output in order to start the sequence without autosampler?
- In case the injection is performed by some controlled valve or instrument (typically EAS analyzers) not supporting the Injection marker by digital output, the common solution when using DataApex A/D board is to interconnect the digital output set for Ready Out signal directly to the digital input set for Start In command. The digital input on the instrument starting the next run will be connected in parallel. With controlled valve, the injection is then performed during the actual run by switching the valve (or valves) from Clarity Event Table.
Events must be set in the Event Table accessible from Method Setup dialog.
- In case no A/D board is used and the devices are not offering those digital inputs and outputs (for example with Agilent GC 5890), the suggested workaround will be to use the DataApex Virtual Digital Input Output Loop (auxiliary device, requires A24 LC or A23 GC Control license) to simulate the input/output needed - (those are virtually interconnected).
For more detail info refer also to Clarity Getting Started manual, chap. 6.