How to determine non-volatile hydrocarbons according to DIN H53 (ISO 16703)?


The recommended procedure for H53 method integration in Clarity:

  1. use the same Peak Width and Threshold as in standard integration. Do not attempt to increase the Peak Width - this parameter causes a bunching of data as a side effect.
  2. apply Baseline - Together to the whole chromatogram
  3. apply Baseline - Forward Horizontal to the whole chromatogram
  4. mark all the peaks between C10 and C40 as a group
    (the group boundary lines may exceed into the C10 and C40 peaks, only fully contained peaks will be included)
  5. amend the peak starts and ends by manual peak tools if necessary for the C10 and C40
    (use the Peak - Both, with the A time corresponding to the respective C10 or C40 time and B time positive or negative, respectively. The times are relative, i.e. will be shifted accordingly in other chromatograms).
  6. create calibration file for C10, C40 and Group A peaks.

To subtract a baseline, in the Method Setup - Advanced dialog, set the baseline chromatogram and in the Matching set No Change. The baseline file should be acquired on stabilized system and preferably without injection of solvent or at least with lower volume of the solvent - otherwise negative artifacts will appear, which will interfere with the correct baseline setting in Clarity.

There is a condition that must be met:

The drawn baseline must not cross the chromatogram line (the negative areas arising do not have any physical meaning).

When the chromatogram is integrated appropriately, use the Method - Save as Template command from the Chromatogram window to save the method as a template method, thus it can be opened on Instrument and used for further measurements.

Example of evaluated chromatogram

chromatogram din 53

Sample data for Clarity

  • DIN-H53.zip - zipped chromatograms method and calibration.
  • Din-H53-processed.zip - zipped Clarity Project to load from Clarity. Unzip it and use Archive/Restore function in Clarity.