Reference Guide > Sequence

Sequence

This Chapter describes window which is used for consecutive measurement of multiple samples. Open the window using the icon or the Analysis - Sequence command from the Instrument window.

Sequence

The window includes a toolbar, sequence table and status bar.

Table description

Each line defines the settings for one or more injections. To process several vials from a single line enter a different value for the initial = start (SV) and last = end (EV) vial. The I/V parameter indicates that there will be multiple injections taken from a single vial.

Status

Status of the row in the sequence.

Indicates a row with one or more errors, such row is disabled from running.

Indicates a row with one or more warnings, measurement will still be carried out.

Point mouse cursor over the icon in the Status column to display a tooltip with detailed description about the injections - it displays how many were measured, skipped, aborted and how many are remaining on the specific row.

Note:

Analysis will be marked as finished after the analysis time elapsed. Note that the instrument method may be still running.

List of possible states of sequence rows

Status State Description
Ready No analysis has been carried out from this row. Measurement will be carried out.
Ready No analysis has been carried out from this row. At least one vial has been skipped. Rest of the vials will be measured.
Ready Analysis has been stopped during acquisition. At least one chromatogram has been measured. Rest of the vials will be measured.
Running Analysis from this row is being carried out.
Completed All analyses on this row have been measured.
Partially completed Apart from the vial(s) where the Skip Vial command has been used, all analyses on this row have been measured.
Partially completed Command Skip Vial has been used on all vials. No more measurement will be carried out from this row. If icon changes color to green and back to original when (un)checking Run checkbox then there are vial(s) that still can be measured.
Partially completed Analysis has been stopped during acquisition. At least one chromatogram has been measured. Run checkbox is unckecked and measurement will not continue unless checking the Run checkbox.
Disabled Row that has been omitted from the sequence. Caused either by an error on the row or by unchecked Run checkbox.

 

Already measured rows will be blocked against changes (dimmed) in all columns except for Run, Sample Type, Lvl, Report Style and Postrun functions, columns which can be changed for reprocessing.

If there is a chromatogram resulting from this row, small triangle will appear in the icon - e.g. (currently measured row with at least one already measured chromatogram). Left mouse click on the triangle will reveal option to open the chromatogram(s). See Fig "Open chromatograms in overlay".

Open chromatograms in overlay

It is possible to open one specific chromatogram or open all measured chromatograms in overlay.

Selected row(s) will return to their initial Status (all fields green) in the following cases:

  • Running of the sequence (by Run command, the icon or F5 or Ctrl + Q shortcut) and clicking Reset button in the displayed dialog.
  • Using the Edit - Reset Status command (Ctrl + E).

Note:

A text description will be printed in the printouts instead of colored tags in the Status column.

Run

Selects whether the particular line in the Sequence Table will be measured. The column corresponds to the Run Lines field in Sequence Options dialog. Working with Run column is more suitable for discontinuous selection of lines or for exclusion of individual lines from measurement.

SV

Number of the first vial used in the processing of the given row.

EV

Number of the last vial used in the processing of the given row. Its number must be the same or higher than the number entered in the SV field. The difference between the end and start vial defines how many vials will be processed by selected line.

If Options - Format - Automatically setting has been applied, then for more than one vial or injection, the File Name pattern will automatically be verified and corrected to ensure that it is in the correct format. In other words, %v and %i variables will be added to the pattern.

If automatic formatting has been applied, the variables in the filename will be supplemented with zeros from the left in order to correspond with defined number of injected samples (e.g. names will be supplemented by numbers ranging from 001 to 100 for SV 1 and EV 100).

Note:

Some autosamplers may support alphanumerical or graphical setting of the SV or EV parameter. In such case, an arrow will appear in the right side of the given field, and clicking it will open particular Select Vial dialog.

I/V

Indicates how many injections will be processed from each vial based on this line in the Sequence Table.

If the Format - Automatically item from Sequence Options dialog has been activated, then, for multiple vials or injections, the File Name pattern will automatically be verified and corrected to ensure that it is in the correct format. In other words, the %v and %i variables will be added to the template.

Sample ID

The sample identification field will be transferred to the headers of all created chromatograms. Maximum number of characters in this field is limited to 64. The parameter corresponds to the Sample ID field from the Single Analysis dialog.

This field serves for information only. After clicking on the cell in this column the icon will appear. Using it invokes the list where you can pick from the available variables for composing the name. Variables that can be used are described in the Chromatogram File Name section of the Single Analysis dialog.

Sample

Sample description - will be transferred to the headers of all created chromatograms. Maximum number of characters in this field is limited to 64. The parameter corresponds to Sample field from the Single Analysis dialog.

This field serves for information only. After clicking on the cell in this column the icon will appear. Using it invokes the list where you can pick from the available variables for composing the name. Variables that can be used are described in the Chromatogram File Name section of the Single Analysis dialog.

Comments

Enable to add user comments. Text is editable directly in the cell or after clicking on dropdown arrow of the icon in Comments dialog. Comments column is hidden and empty by default. Sample comments are saved with the chromatogram and can be displayed on Measurement Conditions tab and in Print Preview.

Note:

Comments in the already measured chromatogram are also editable from Measurement Conditions tab.

Sample Amount

The value is used in calibration calculations of the ESTD and ISTD methods. When using the ISTD calculation, enter the sample amount without the internal standard. Enter values in units used in the calibration file (as defined in the Units - Compound field of the Calibration Options dialog). The default value is 0. The parameter corresponds to the Amount field of the Single Analysis dialog and the value will be transferred to the chromatogram header.

ISTD1 Amount

The amounts of the internal standards for the ISTD method. Other columns for entering amounts of ISTD2 - ISTD10 are hidden by default. This value has to be entered in the same units as those used in the calibration file (as defined in the Units - Compound field of the Calibration Options dialog). The default value is 0. The parameter corresponds to the homonymous field from the Single Analysis dialog and the value will be transferred to the chromatogram header.

Sample Dilut.

This field holds the sample dilution factor - its value is used to multiply each calibrated amount in the Amount column of the Result Table. The default value is 1. The parameter corresponds to Dilution field in the Single Analysis dialog and the value will be transferred to the chromatogram header.

Inj. Vol.

Defines the volume of injection. The response of all compounds in a calibration or when using calibrated calculations is modified by the ratio of this value and Default Injected Volume value from the calibration file. This parameter corresponds to the analogous field from Single Analysis dialog and its value will be transferred to the chromatogram header. The units are set in the Instrument Units dialog (described in the chapter "Right section of System Configuration dialog").

Caution:

When using a directly controlled autosampler this value will define the injection volume! The station then automatically verifies whether the entered volume corresponds to the current autosampler setting.

When the value set in this column is 0, no correction to the Default Injection Volume value will be made. However, when Inj.Vol. of 0 is entered into the sequence when using a directly controlled autosampler, a warning will be given.

AnalysisUserVar1 - AnalysisUserVar3

Sets numerical value of User Variables (up to 3 per row). User Variables can be used for User Column calculations. Columns AnalysisUserVar1 - AnalysisUserVar3 are hidden and set to value 0 by default. Name of the columns can be changed in Setup Columns... through Custom Name line. If the field is left empty, default name AnalysisUserVar1-AnalysisUserVar3 would remain filled in.

Note:

User Variables columns can be edited after sequence end and reprocess in the next batch.

Caution:

Users who do no have the right to Edit Method in the User Accounts dialog cannot edit AnalysisUserVar1 - AnalysisUserVar3; fields will be grayed out.

File Name

Defines the chromatogram name pattern. The pattern may use a combination of fixed text and variables (usually entered in the form of % and a character). The same variables may be used as in the Single Analysis dialog, plus %v and %i variables for automatic calculations of vials and injections when measuring multiple analysis from one line.

After clicking on the cell in this column the icon will appear. Using it invokes the list where you can pick from the available variables for composing the file name. You can also enter a subfolder in the chromatogram name. Variables and how to enter the subfolder name are described in the Chromatogram File Name section of the Single Analysis dialog.

It is possible to include also the name of a subfolder in the name to store chromatograms into subfolders of the Data Subdirectory. E.g. the MySubProject\MyFileName in the DEMO1 project will result in the MyFilename.prm stored in the C:\CLARITY\Datafiles\DEMO1\DATA\MySubproject folder. If the subfolder doesn't exist, then it will be created when the chromatogram will be stored.

Note:

If variable %n is being used, it will be independent from the variable using the same name in the Single Analysis dialog.

Sample Type

Sets the type of the sample being measured. The possible options are:

Unknown - denotes an unknown sample.

Standard - specifies the calibration standard. Samples marked as Standard must have the calibration level filled in the Lvl column. Chromatograms created on the sequence rows marked as calibration standards will be stored in the calib subdirectory instead of the data subdirectory.

Bypass - this Sample Type type allows for performing an analysis in the Active sequence with controlled autosampler without actually injecting a sample. This may be useful for system clean-up etc..

Blank - in fact a calibration standard with no amount of added sample. The Blank is thus one point of a calibration curve (with the Amount of 0).

Caution:

When measurement of recalibration standard is finished, the calibration file being recalibrated is updated by the sample responses and saved. In case there are any unsaved changes within the calibration file at that moment, those changes are discarded.

Lvl

Indicates the number of the calibration level when the resulting chromatogram is to be used for automatic recalibration when the Sample Type column of the sample is set to Standard. The file has to be set as the calibration standard in the Sample Type column and a proper calibration file has to be set in the current method as a prerequisite for automatic recalibration.

Method Name

Indicates the name of the method governing all analyses set on a given line. The active template method name from the Instrument window will automatically be inserted in the first line of the new sequence table.

Note:

If you point the mouse cursor to the title of the method, the yellow tooltip will open, in which the title of the method including the entire path will be displayed.

Report Style

Sets the report style to be used for printing. If no name has been entered, no printing on a given row of the Sequence Table will be performed. After clicking on the cell in this column the icon will appear. Using it opens the Insert File Name dialog for specifying the print method.

Caution:

Print style cannot be selected while a sequence is running.

If you point the mouse cursor to the title of the print style, the yellow tooltip will open, in which the title of the print style including the entire path will be displayed.

Postrun functions

The commands Open, Open Calib., Print, Print to PDF, Export Data, Export AIA, Export TXT, Export EZChrom, Export Multidetector, Run Program, Program to Run and Parameters generally correspond to analogous items in theSingle Analysis - Post Run Settings tab (for more details see also chapter Single Analysis - Post Run Settings) . All of these columns contain checkboxes, with the exception of the Program to Run column (corresponding to the Program to Run field of the Single Analysis - Post Run Settings tab) and the Parameters column.

Note:

When there is a printing set in the Postrun functions which includes the chromatogram or results, the Open chromatogram Postrun function has to be selected too. Only chromatograms opened in the Chromatogram window can be reported.

When the sequence is running, the postrun switches in the Instrument window will be locked to the state that has been set on the currently running row.

Note:

In the implicit layout only the Open, Open Calib. and Print columns are visible, the rest is hidden. Use the Edit - Setup Columns… command to display the desired columns.

Include in SST

This checkbox allows user to set whether the particular chromatogram will be included in the SST calculations or not.

Stored Calib.

This checkbox causes the created chromatogram to be opened with the recent stored version of the calibration file, as opposed to the linked calibration when not checked. The checkbox corresponds to the Open with stored calibration checkbox in the right part of the Chromatogram - Results tab.

Close All

This checkbox allows user to close all currently opened chromatograms. Opening of the chromatogram measured in the same line of sequence is not influenced by this setting, it follows setting of Open checkbox only.

Note:

To resize a column, point mouse cursor on the edge of the column to display , left mouse click and drag to a desired width. All columns, apart from Status, can be resized.

Status Bar

The status bar provides the following information:

State

If the sequence is running, the runtime of the actual sample is displayed. If the sequence is not running, status from the Instrument window in the form of <short status> - <long status> is displayed.

Vial No.

The vial number / injection number from a given vial.

File Name

The name of the chromatogram currently being measured.

Active (Pasive)

Active/passive sequence flag.

Auto (Manual)

Chromatogram name control flag showing the settings of the Format section in the Sequence Options dialog.