How to try the DHA demo project

In this chapter, you can find information on how to try the DHA Extension using the DHA demo project for the first time.

Step 1

In the System Configuration window (menu Clarity - System…), select the DHA option in the Instrument Type Setting dialog, for example on Instrument 1.

For more details see also chapter Installation.

Step 2

After saving and closing the System Configuration window, open the appropriate Instrument. Now, open the DHA demo project: in the Login Dialog, select DEMO_DHA in the Select Project drop-down list and click the OK button.

Step 3

Open the Chromatogram window (it can be opened by clicking on the menu Window - Chromatogram). In the lower part of the Chromatogram window, you can see the new DHA tab. Open the chromatogram N_PARAFFINS_FOR_ASTM6730.PRM using the File - Open Chromatogram… menu. The file is located in the DEMO_DHA\Data folder in your Clarity installation directory (by default C:\CLARITY\DataFiles). This file is the calibrated (known) sample, including the n-Paraffins standard.

Step 4

Click the DHA tab, and on the right side of the pane, you will see the DHA settings. There you can set the norm or create a new DHA Calibration. The current settings are saved in a special .DHA file, which allows you to use the DHA settings on multiple Chromatograms. Click the Open… button to browse the Clarity Installation directory (by default C:\CLARITY\DataFiles). In the DEMO_DHA subdirectory, select the ASTM6730.dha file. DHA Method will be loaded, and the Chromatogram which is activE will be automatically recalculated when you choose different norms or parameters.

For more details see also chapter DHA Settings .

Step 5

In the DHA Settings pane, select the norm, for example ASTM D6730 (official). This norm will be used when creating the DHA Calibration. Click the Create DHA Calibration button, and the Calibration window, including the new DHA Calibration, will be displayed. Save the calibration file. If you link this calibration to an unknown sample (chromatogram) in the DHA Extension, you will see the calculated result of specific groups.

Step 6

Close the Calibration window, and in the Chromatogram window, open an unknown sample from the Demo_DHA project, for example the ASTM6730_455.PRM. Click the DHA tab, and in the lower part of the DHA Settings, you will see that this chromatogram has already linked a calibration file ASTM6730_C5-C15-RI. Note that this is the same function as the Calibration File field in the Results tab of the Chromatogram window. You can change it to the calibration created in Step 5.

Step 7

In the Result Table sub-tab of the DHA tab, you can see the calculated results of compounds identified in the chromatogram. Click the Group Result Table, where the compounds are calculated in a pivot table. It contains the sums of all compounds per group or carbon number.

If there is a compound in the Result Table that is not in the selected norm in the DHA Settings, the Status column displays a warning. The norm requires that all the compound names are the same as in the norm tables. If you need to link a compound name from the chromatogram with a matching compound name from the norm, click the Edit Link Table… and in the displayed dialog, link the compound name from the chromatogram with the name in the norm.