Setting up a method

The method essentially contains information on how the analysis will be performed, how the resulting signal will be processed and what events will be triggered and when.

Any method can be created or edited in the Method Setup window. To use the method for measurement it has to be sent to the instrument and for sequence measurement method has to be set at each row.

Template Method vs. Chromatogram Method

• The sent method is used as a template for new chromatograms and its contents are copied into the chromatogram file after acquisition or batch file reprocessing. You can open the different sections of the sent method setup from the icons in the Instrument window or with the command Method - ...... on the toolbar.
• The changes to the chromatogram method do not affect the template method and they are performed in the lower pane of the Chromatogram window.
• The calibration file is linked to the template method and to the chromatogram method by its name.

Method Setup window

• The title of the Method Setup dialog displays a method that is currently opened. If you make any changes, the method becomes (MODIFIED).
• The upper part of the Method Setup dialog displays a set of icons with which it's possible to create a new method, open existing method, save method, save method as, open Report Setup, open method audit trail, send method by e-mail or open help.
• Upon pressing the OK button it will be automatically saved and Method Setup dialog will close.
• Tabs shown in Method Setup window are dependent on devices actually configured on the Instrument, e.g. MS Method, PDA Method, LC Gradient can be only visible when proper instrument type and device is set.

Send Method

• Only a method that is saved can be sent.
• Pressing the Send Method button will result in two actions:
  • Method will be sent to all connected hardware and thus displayed in the information table of the Instrument window - just as before.
  • Method will be set as Method for Single Analysis - you can start the single analysis form the instrument window using the icon.
• When measuring sequence, each row can have different method. It is set while creating or editing the sequence tab in the Method Name column.

Method Development

Method currently used for acquisition or present on already locked row of the sequence can't be edited, and can be viewed in read-only mode only.

When developing a method, you can configure the following settings among others:

1. Set the measurement conditions.

   Here you can disable or enable Autostop and set the run time for the analysis ⓐ as well as configure the external signal start and stop settings ⓑ (default setting Start Only should be used in majority of applications). Autostop can be set within interval 0.2 - 9999 min, such value is not influenced and does not influence any device specific time program settings on different tabs (like LC Gradient or GC). When time defined in Autostop passes, acquisition is finished, chromatogram is created, remaining time from time programs will be spent in so called Control state.

   You can also describe some parameters of the method and add a note ⓒ. Such information is purely informative and do not influence analysis in any way. They are saved into the resulting chromatogram and are showed in the Chromatogram window.

2. Set the Data Acquisition parameters.

   It allows you to select, enable/disable the detector signals ⓐ and to set measurement parameters e.g., the signal range and sample rate ⓑ. At any time at least one detector has to be Enabled.

   Layout of this tab, together with possible parameters to be set, are dependent on the configured devices (control modules) on the Instrument.

3. Set the Signal Integration parameters.

   When using a multi-detector configuration, there is an integration table for each detector (i.e. signal), thus every resulting signal can be integrated in a different way.

   Every resulting chromatogram acquired with such method will be integrated automatically accordingly the integration tab.

   If you aren’t able to edit tab parameters the current detector probably isn’t Enabled.

 

4. Set the calculation options.
   • You can create New... calibration file or Set... the one created previously ⓐ and configure the different settings related to it.
   • When calibration file is set, resulting chromatograms will be calibrated according to it and calibration standards will be used to automatically re-calibrate the attached calibration file.
   • Clone... ⓑ button will create a copy of a current calibration file. In the following Save As dialog you can select the name and location of the new calibration file. The copy will then be linked to the method.
   • If it is desired to have no calibration in the method you can use the None button ⓒ.
   • In this tab you can also choose how you want the results to appear in the results ⓓ and set calculation type ⓔ .
   • Name of calibration clone ⓕ must be set here to use calibration cloning in sequences, refer to chapter Calibrating using clone on first recalibration.

5. Configure the settings in the additional tabs.

   Method Setup also has tabs specific to currently configured devices and Event Table tab, used for configuring what events will be triggered and when e.g., shutting down Clarity after the sequence is finished.

   However, there are some limitations:

   • Event Table is for specific purposes, not needed for standard analyses.
   • Does not have control against infinite loops.
   • Must be filled per row, Fill Down/Fill Series can't be used in Event Table.