Creating a new calibration

This chapter covers creating a calibration file. You should have at least one integrated measured standard to be able to fill in the desired peaks into the newly created calibration.

1. Open the Calibration window: choose Window - Calibration in the Instrument window or click .

2. Create a new calibration file: select File - New or click ⓐ .
3. Open the Calibration Options dialog: choose Calibration - Options… or click ⓑ.

4. Fill in the units in the Compound Units section ⓒ to suit your analysis conditions.
5. Set the Mode to Calibrateⓓ .
6. Set the Calibration option to Automatic to add the peaks without modification or Manual to modify them one by one ⓔ.
7. Open integrated chromatogram of a standard: choose File - Open Standard… or click on ⓕ in the Calibration window.
8. Add peaks belonging to the compounds of interest from the chromatogram of the standard to the calibration file.

   Note:

   Select Calibration - Add All or click on to add all integrated peaks or the Add Peak / Add Group icons to add specific peaks ⓗ. Regardless of the set Current Level ⓖ the peaks will be added to the first free level.

9. If you selected Automatic calibration:
   • Name the peaks identified in the Calibration Summary Table ⓘ by their retention times by typing the Compound Name for each peak. No compound name may be used more than once.
   • Fill in the Amount ⓙ for each compound into the Calibration Summary Table.

10. If you selected Manual calibration: Fill in the Amount, the Compound Name and set any other parameters related to the peak on the Calibration - Add Peak window. This window will open once for each one of the peaks processed.

11. Save the calibration file - File - Save or click .