Calibrating using clone on first recalibration

Option Clone on first recalibration sets the sequence and calibration to the Safe Calibration Usage mode. This option will create a clone (copy) of calibration defined in the method upon completing the first row of the sequence. Cloned calibration is attached to each new chromatogram produced by the given sequence.

  1. Open the Method Setup dialog - Calculation tab. Select Method - Calculation from the Instrument window.

  1. Click on Set... button and select a calibration file to be used during cloning at first recalibration.

    Note:

    This calibration will remain unchanged as newly created clone of the calibration will be used with new responses.

  2. Create a custom name for the calibration files in Calibration Cloning in Sequence as explained in Creating customized file names automatically.

    Note:

    The name of the final calibration file will match just the content of this field - if you wish to include the name of the template calibration, include the name in this field again (e.g. "test - %s %L %R").

  3. Follow the steps in Creating and running a sequence to create your sequence based on the example below.

  1. Click to open the Sequence Options Dialog .
  2. Select Clone on first recalibration and click OK.

  1. Run the sequence as explained in Creating and running a sequence.

Note:

The measured sequence can be reprocessed using Batch. For more info see the topic Reprocessing whole sequence while using calibration cloning.