In order to have the integration of routine samples automated as much as possible it is necessary to optimize integration parameters and then save them to the method that is used for measurements. Two integrations algorithms (Wave and Legacy) with slightly different approach are available, if you are not able to achieve satisfactory integration in one of them try using the other one. Integration Algorithm can be changed on Integration tab in Method Setup/Chromatogram.
- Open one typical chromatogram for your analysis.
- Set Integration Interval as needed. More details are in Setting Integration Interval topic.
- Set Global Peak Width parameter. Choose the narrowest peak which should be integrated in your chromatogram and select his start and end. This setting is used for calculation of Global Bunching.
- Set Global Bunching parameter. This is a filter which is calculated based on Global Peak Width and sample rate.
- Alternatively, for chromatogram with high noise, it is possible to use other filters - FFT Filter, Savitzky-Golay Filter or Moving Average Filter.
- Set Global Threshold parameter. This is influenced by the Global Bunching or other filter set previously. Select an interval containing only noise, not peaks. A more detailed description of the global parameters is covered by topic Modifying Global parameters.
- (Optional) Set Detect Negative parameter. This has two potential uses, firstly when you want to integrate also negative peaks. Secondly it may help when signal has inconsistencies such as dips. This will prevent the integration algorithm from placing peak starts/ends into them.
- Set Global Baseline Slope parameter. Change this parameter after you have all the peaks integrated, it servers to optimize their start/end positions.
Note:
Global Slope is available only in Wave Integration Algorithm.
- If you still aren't satisfied with the integration there are another possibilities for manual changes.
- Try to use Interval parameters such as Baseline Together, Baseline Valley, Forward Horizontal etc..
- Or try to use Peak parameters such as Peak Start, Peak End, Peak Both, Add Positive etc..
Note:
Add Positive operation should be used as a last resort because is prone to every Retention time shifts. Peak Start and Peak End should be preferred parameters as they are defined relative to peak apex.
- When you are happy with the integration. Save method as Template and use it for further measurements of these sample types.
- If you have already measured some chromatograms and want to use this integration in them you can do it using Batch - Reprocess by Method.