Multiple Broad Integral Calibration

The standard to be used will have a table detailing the % Cumulative Weight Fraction (Percent [%] in Clarity) and Molecular Weight (M) values supplied by the manufacturer of the standard. Multiple Broad Peaks can be used subsequently to extend the area of calibration reliability.

GPC Calibration (Broad Integral)

After selecting the calibration type the Percent [%] and M parameters must be entered manually. To paste a table of desired parameters correctly, select only the cell where the table should start. When more cells are selected, the only first row of the table will be copied to the Calibration Table.

Standard chromatogram should be opened next. The Set Broad Peak icon will recalculate the RT values according to the data entered into the % Cumulative Weight Fraction field using the peak being calibrated. Operation is performed only for rows with Std. No. value equal to current Std. No. indicated in the Window toolbar of the Calibration window.

The % Cumulative Weight Fraction and Molecular Weight values are common for all signals. If this type of calibration is to be used for multi signal chromatograms, the Detector Delay function should be used to put the signals on a common time base.

Note:

The last Broad Peak is temporarily remembered and used in subsequent recalculations. Current Broad Peak is indicated in right lower corner of the Calibration window.

Note:

When a new compound is manually created in Calibration Table there are pre-filled default values 0 for M, Log M, Log M Adjusted, Max RT, Flow Marker RT, RT Corrected, Deviation and Intrinsic Viscosity columns; default values 1 for FR Correction and Source Chromatogram columns. Column K is pre-filled with default value 14,10 and column Alpha is pre-filled with default value 0,70.