How to set PDA method
This section deals only with method setup related only to PDA options. For a method setup see "Setting up a method"
- PDA Method tab ① is available only when PDA Extension is configured on the given instrument.
- From the drop down ② box select PDA Spectrum - if chromatogram contains more than one (typically DAD and FLD).
- For the Peak Purity Options there are several settings which can restrict the evaluation of peak purity. It can be restricted based on the Restrict Wavelength Range ③ check-box and filling the range of wavelength in which it will be evaluated.
- Another restriction can be made using the Absorbance Threshold ④. This determines which part of peak will be used. For 0% spectra from entire peak will be included in calculation. For 5%, spectra which has less than 5% of absorbance compared to spectrum in apex will be excluded from calculation etc.
- Used Points ⑤ specifies the number of points peak purity will be evaluated, either from the All the points or from the Five most significant.
- In the Library Search Options ⑥ select criteria according to which the Search in Library command, described in see this procedure, will be performed.
- To add another PDA library to search in, click the icon ⑦ and then click , from the dialog choose a library.
- List of libraries that will be searched in is listed ⑧. Alternatively enable/disable the search for specific library using the check-box.