How to set PDA method

This section deals only with method setup related only to PDA options. For a method setup see "Setting up a method"

  1. PDA Method tab is available only when PDA Extension is configured on the given instrument.
  2. From the drop down box select PDA Spectrum - if chromatogram contains more than one (typically DAD and FLD).
  3. For the Peak Purity Options there are several settings which can restrict the evaluation of peak purity. It can be restricted based on the Restrict Wavelength Range check-box and filling the range of wavelength in which it will be evaluated.
  4. Another restriction can be made using the Absorbance Threshold . This determines which part of peak will be used. For 0% spectra from entire peak will be included in calculation. For 5%, spectra which has less than 5% of absorbance compared to spectrum in apex will be excluded from calculation etc.
  5. Used Points specifies the number of points peak purity will be evaluated, either from the All the points or from the Five most significant.
  6. In the Library Search Options select criteria according to which the Search in Library command, described in see this procedure, will be performed.
  7. To add another PDA library to search in, click the icon and then click , from the dialog choose a library.
  8. List of libraries that will be searched in is listed . Alternatively enable/disable the search for specific library using the check-box.