Creating a GPC calibration
To be able to create a GPC calibration, you need to have a measured and integrated GPC chromatogram of a standard sample and the instrument type of Clarity must be set to GPC. The GPC mode can be toggled on/off by selecting the Setting - GPC Mode in the Instrument window.
- Open the Calibration window: select Window - GPC Calibration in the Instrument window or click on .
- Create a new calibration file: select File - New or click on ⓐ.
- Following dialog GPC Calibration Options will show up. Here you can setup various options and Calibration Types.
Caution:
Once selected, the Calibration Type can't be changed later. More on GPC Calibration Options can be found in the GPC Extension manual, accessible on www.dataapex.com.
- Click OK to save the calibration. To fill calibration name open the File - Save As dialog window.
- Open integrated chromatogram of a standard: select File - Open Standard… or click on ⓑ in the Calibration window.
- Add peaks in the chromatogram of the calibration standard to the calibration file: select Calibration - Add All or click on ⓒ (if you have multiple peaks in your chromatogram) or select Calibration - Add Narrow Peak or click on ⓓ to add desired peak from your standard.
Note:
If you have multiple chromatograms of standards, you can repeat these steps to add the desired peaks: open the standard and click Add Narrow Peak, then open another standard and click Add Narrow Peak, repeat for all standards. The number in field ⓔ is connected to the used standard and is not connected to the concentration level. Setting a peak on already used number will overwrite the values with a newly added one.
Note:
When using any type of Broad calibration, Add Broad Peak will be enabled instead of Add Narrow Peak.
- Fill in the appropriate molecular weight values for the respected peaks into the M column ⓕ.
- Save the calibration file: from File - Save or click on ⓖ.