Creating a GPC calibration

To be able to create a GPC calibration, you need to have a measured and integrated GPC chromatogram of a standard sample and the instrument type of Clarity must be set to GPC. The GPC mode can be toggled on/off by selecting the Setting - GPC Mode in the Instrument window.

  1. Open the Calibration window: select Window - GPC Calibration in the Instrument window or click on .
  2. Create a new calibration file: select File - New or click on .
  3. Following dialog GPC Calibration Options will show up. Here you can setup various options and Calibration Types.
  4.  

Caution:

Once selected, the Calibration Type can't be changed later. More on GPC Calibration Options can be found in the GPC Extension manual, accessible on www.dataapex.com.

  1. Click OK to save the calibration. To fill calibration name open the File - Save As dialog window.
  2. Open integrated chromatogram of a standard: select File - Open Standard… or click on in the Calibration window.
  3. Add peaks in the chromatogram of the calibration standard to the calibration file: select Calibration - Add All or click on (if you have multiple peaks in your chromatogram) or select Calibration - Add Narrow Peak or click on to add desired peak from your standard.

Note:

If you have multiple chromatograms of standards, you can repeat these steps to add the desired peaks: open the standard and click Add Narrow Peak, then open another standard and click Add Narrow Peak, repeat for all standards. The number in field is connected to the used standard and is not connected to the concentration level. Setting a peak on already used number will overwrite the values with a newly added one.

Note:

When using any type of Broad calibration, Add Broad Peak will be enabled instead of Add Narrow Peak.

  1. Fill in the appropriate molecular weight values for the respected peaks into the M column .
  2. Save the calibration file: from File - Save or click on .