Recalibrating a calibration

You can modify an existing calibration by reloading peaks within one specific level using the option Recalibrate.

1. Open the Calibration window: choose Window - Calibration on the Instrument window or click .

2. Open the calibration file: choose File - Open… or click ⓐ.
3. Open the calibration standard with which you want to recalibrate: select File - Open Standard… or click ⓑ .
4. Open the Calibration Options dialog: choose Calibration - Options… or click ⓒ.

5. Set the Calibration option to Automatic to add the peaks without modification or to Manual to modify them one by one ⓓ.
6. Set the Mode to Recalibration ⓔ.
7. Select how and whether the new values will be added in the Calibration Options dialog.
   • Choose Replace or Average option to decide what to do with new response values ⓕ.
   • Recalibration Search Criteria defines how much the original and new values can differ for the recalibration to be performed ⓖ.
8. Select the Level you wish to recalibrate ⓗ.
9. Add peaks to be recalibrated from the calibration standard to the calibration file on the Calibration window using Calibration - Add Existing or clicking on ⓘ.
10. If you selected Manual calibration:
• Fill in any parameters related to the peak in the Calibration - Add All window. This window will open once for each one of the peaks processed.

11. Save the calibration file: choose File - Save or click on .