Sequence window

Analysis Procedure: Step 1 -> Step 2 -> Step 3 -> Step 4 -> Step 5

  • In the main Clarity window, open Instrument 1 (With the label My GC + AS).
  • In the displayed Login dialog with the pre-selected Administrator user, click the OK button.
  • Use the Sequence button in the Instrument window to enter the Sequence window.

    • Note:

    Sequence window contains similar setting to Single Analysis dialog, but they are organized into separate columns.

Sequence window

  • Look at the Sequence Table. Each row of this table defines one or more analyses, depending on fields SV (Starting vial), EV (Ending vial) and I/V (Injections per vial) ①. As it can be seen, the first four rows each present a single measurement (SV and EV is the same, I/V is 1), while row 5 represents eight analyses (SV is 5, EV is 8, thus measuring 4 samples from 4 successive vials, and I/V parameter is 2 - each sample will be measured twice).
  • Also, note that in the Sample Type and Lvl fields ② the first four samples are marked as standards on levels 1-4. These will be used for automatic making of the calibration (or its recalibration, if there already were any data in the calibration).
  • The Method Name column ③ sets the template method used for measuring the sample. The Report Style column ④ sets the print style used for reporting the measurement. Each row can have its own template method and report style; it is thus possible to measure according to several template methods in one sequence.
  • In the File Name column ⑤…, the name of the resulting chromatogram file is specified. It is possible to use variable parameters to form the chromatogram filename, for example %Q means that the file name will use the text from the Sample field. It is possible to combine several of these variables with plain text or symbols to create a unique file name for each chromatogram. The complete set of available variables can be seen after clicking in the field and selecting the icon.
  • To check the sequence, click the icon ⑥. Clarity station will keep all symbols at the beginning of the row green () meaning the row is ready or issue an error/warning message listing what should be corrected on which row to be able to proceed.

Note:

For demonstration purposes only, try to make a mistake and check the sequence once more. For example, on row number 3, change the text in the Sample column to Std_1, you can immediately see that a warning sign appeared on the corresponding rows - 1 and 3. After pressing the icon, warning message appears telling that there are two rows which would produce chromatogram with the same file name. Holding the mouse above either field will display the tooltip with the cause of the problem. Set the sequence back to its original state and continue to the next step.

  • Start measuring the sequence using the icon ⑦. The state of the ACTIVE sequence will change to WAITING FOR INJECTION and as soon as the Start signal from the autosampler is detected, the measurement will start.

Note:

Even if the autosampler is not connected, Clarity Demo will get the Start signal, thus starting the sequence. However, it is not possible to give its own demo data for each chromatogram, so all chromatograms would be the same. All files are already prepared for you. You may stop or abort the sequence now or later either from the Data Acquisition window or directly from the Sequence window.

  • After the first row of the Sequence table (controlling one analysis) is measured, the Instrument will once again switch to the WAITING FOR INJECTION state and the autosampler will start a new measurement by sending the Start signal. Stop the sequence from the Data Acquisition window or Sequence window at any time by pressing the Stop button (single-click means that the currently measured Chromatogram will finish and the sequence will stop subsequently, after second click the sequence will stop immediately). All data measured will be saved. Or abort the measurement with Abort button (does not produce any chromatogram ).
  • Already measured rows will change Status from green field () to icon with small chromatogram (). If there is a chromatogram resulting from this row, small triangle will appear in the icon - . Left mouse click on the triangle will reveal option to open the chromatogram(s). You can click on the name of the chromatogram to open it or select option to open all chromatograms in overlay.

Note:

It is possible to edit the sequence even during the measurement.

  • On the right side of the Sequence Window you can check at each row whether corresponding chromatogram should be opened, printed or loaded into the calibration window.

Note:

There are many more hidden columns, they can be displayed by right-clicking the sequence table and selecting Setup Columns. In the following dialog move columns that should be displayed to Show Columns list.