Linking the calibration to a chromatogram

  • Any chromatogram can be linked to a calibration file, thus automatically giving calibrated results. In the Instrument window, use the Chromatogram icon to open the Chromatogram window.
  • Use the Open Chromatogram icon to open chromatogram data based on the calibration you have just created. Use the SAMPLE_VIAL_6-1.PRM file saved in the default directory. Other files in the directory are uncalibrated too, but they will be used later.
  • The data are uncalibrated and no information about the names of individual compounds is available, peaks in the Result Table are just described according to their retention times. To change this, the appropriate calibration should be linked to this chromatogram.
  • Select the Results tab and look at the section on the right side of the screen. Use the Set... button in the Calibration File (Peak Table) section to select the calibration file (calibdemo.cal) created in the previous chapter. Any peaks present in calibration are now identified with their names.

Note:

In case you skipped the process of making your own calibration, please use the pre-prepared DEMO1.CAL instead of CALIBDEMO.CAL.