Appendix - Mathematical Formulas

Mathematical formulas

  EP/USP USP until 12/2022 JP
Efficiencyi
Assymetryi
Symmetry/ Tailing
Capacityi
Eff/li
HETPi
Resolutioni

Formula for parameter calculation

where:

Description of symbols

Symbol Description Symbol Description
RTi - Component retention time bi - Right half-width at 10% height
Wh/2i - width at half height A05i - Left half-width at 5% height
Wi - Baseline width B05i - Right half-width at 5% height
ai - Left half-width at 10% height TM - Retention time of the unretained solute.

 

RSD [%]

Percentage standard deviation (variation coefficient).

where:

Description of symbols

Symbol Description
n number of validated (loaded) chromatograms
Xi the value of a monitored parameter of the i-th chromatogram
arithmetic mean of a monitored parameter of all validated (loaded) chromatograms