Defining Reference m/z values

You can define Quantification (Quantify On column) and Reference (EIC 1..4 - Reference m/z 1..4 columns) m/z valuesaccording to the needs of your analysis.

When setting the Reference m/z values for the given peak, you can either:

  • enter m/z values for the peaks directlyinto the cell, using either the values you already know, those listed in a table, or those visible in the graph.
  • enter the numbers interactively using the Add Reference m/z icon or menu command while you have a row in the MS Method Table selected; while using the icon/command, the mouse will be locked in the MS Spectra graph and you will select the desired m/z stick. The m/z value of this stick will be filled in the first free EIC - Reference m/z field on that row.

Note:

You can choose which spectral lines will have peak tags displayed in the MS Spectra graph by using the Properties… command from the graph local menu and going on the MS Spectrum tab.

Setting of the Quantification signal is straightforward - select one of the options in the Quantify On column for the given compound (table row). Each compound can be quantified on different signal.