Defining Reference m/z values

You can define Quantification (Quantify On column) and Reference (EIC 1..4 - Reference m/z 1..4 columns) m/z according to the needs of your analysis.

When setting the Reference m/z values for the given peak, you can either:

  • enter the m/z’s for the peaks directly by entering the values you know, which are tabulated or which you see in the graph into the particular cell.
  • enter the numbers interactively using the Add Reference m/z icon or menu command while you have a row in the MS Method Table selected; while using the icon/command, the mouse will be locked in the MS Spectra graph and you will select the desired m/z stick. The m/z value of this stick will be filled in the first free EIC - Reference m/z field on that row.

Note:

You can define the peak tags being shown in the MS Spectra graph by using the Properties… command from the graph local menu and going on the MS Spectrum tab.

Setting of the Quantification signal is straightforward - select one of the options in the Quantify On column for the given compound (table row). Each compound can be quantified on different signal.