Creating of the MS Calibration

To create the MS Calibration file, it is enough to click the Create MS Calibration button on the MS Method pane. Most of the things that users normally fill in in the Calibration window were already filled into the MS Method, so all of these information will be automatically copied into the MS Calibration window. Most of the operations are the same in the MS Calibration window as are in standard Clarity calibration.

Even though MS Evaluation uses a lot of signals, only one calibration compound table is present in the MS Calibration window. The quantification signal changes for each peak depending on what is set as a quantification signal in the MS Method. There is a new Fill All Responses from Quant. Signals button in the MS Calibration window, which will fill in the respective responses from all peaks present in the MS Method to the opened calibration level. The new button is there to cope with the switching quantification signals on different peaks.

To create the MS Calibration:

• On the MS Method tab in the MS Chromatogram window, press the Create MS Calibration button.
• The view will switch to the MS Calibration window.
• Open the calibration standard file as usual.
• Select the correct calibration level and ensure all calibration parameters are set correctly (calibration/recalibration, automatic/manual, …).
• Fill in the particular compound amounts into the Amount column.
• Use the Fill All Responses from Quant. Signals button.

Same as in standard Clarity, you have to save the created MS Method as a template to be able to measure according to it, and connect the calibration file with the chromatogram to see the calibrated results.