Integration of signals in MS-MS

In MS-MS Extension, two types of signals can be integrated: Quantification Signals and Result Table Signal.

Result Table Signal

Result Table Signal serves for filling the Result Table with peaks other than those mentioned in MS Method. Any signal from the detectors configured on the particular Instrument can be selected as Result Table Signal, this is done through navigating to the MS Method tab in the Chromatogram window and changing the Result Table Signal in the respective dropdown. Default value can be changed in the Method Setup dialog when template method is opened, MS Method tab.

To see what is entered in the Result Table Signal's integration table and be able to change it, please:

  • Switch to Integration tab in Chromatogram window.
  • In the drop-down list select Result Table Signal (as defined on MS Method tab). If other signal than the Result Table Signal is selected, integration table will not be shown at all.
  • Apply integration operations as desired.

Quantification Signal

Quantification Signal serves for quantification of compounds mentioned in MS Method. Integration table is unique for each quantification signal. When multiple compounds are quantified on the same quantification signal they will share the same table.

  • Switch to MS Integration tab in Chromatogram window.
  • In the drop-down list select desired compound (defined by m/z ion and the name). Chromatogram will be focused on the compound's peak.
  • Apply integration operations as desired.

Note:

Even if you select Result Table Signal, e.g. TIC also as your Quantification Signal for any of your compounds, the integration table from Result Table Signal will not be used for such Quantification Signal. Each quantification signal has its own integration table.