Peak

Set of commands for operations with peaks. All commands can also be invoked using icons from the Peak toolbar.

All interval operation follows the rule that with Time B = 0 operation will be applied from Time A to the end of the chromatogram. With both Time A and Time B set to 0, operation is applied over the whole chromatogram.

Peak Toolbar

Show Displays hidden peaks on the selected time interval. Current baseline is applied. Available only if Wave Integration Algorithm is used.
Hide Hides all peaks on selected time interval. Baseline remains unchanged. Available only if Wave Integration Algorithm is used.
Start Shifts the peak beginning.
End Shifts the peak end.
Both Shifts the common point of two peaks.
Add Positive Creates a new positive peak.
Add Negative Creates a new negative peak.
Force Peak Name Forces the peak a new name from calibration.
Solvent Peak Marks the peak as solvent peak.
Peak Groups Invokes a dialog for working with groups of peaks.

Invoking this command or the Start icon shifts the beginning of a peak. If this shift would move the peak start ahead of the end of the preceding peak, that end will be shifted to the selected point and both peaks will be separated by a vertical line. If the beginning of the peak before shifting has the same retention time as the end point of the preceding peak and will be shifted closer to the peak apex, the previous peak's end will stay at the original point and both peaks will be separated. The peak beginning may be shifted only within the interval delimited by the apexes of the previous peak and peak being modified.

Shift peak start

Invoking this command or the End icon shifts the end of a peak. If this shift would move the peak end behind the beginning of the following peak, that beginning will be shifted to the selected point and both peaks will be separated by a vertical line. If the end of the peak before shifting has the same retention time as the start of the following peak and will be shifted closer to the peak apex, the following peak's beginning will remain at the original point and the peaks will be separated. The peak end may be shifted only within the interval delimited by the apexes of the following peak and the peak being modified.

Shift peak end

Invoking this command or using the Both icon shifts the common point of two peaks (a valley or the separating vertical line). The point may be shifted only within the interval delimited by the apexes of the both separated peaks.

Shift valley line

Invoking this command or using the Add Positive icon creates a new, positive peak. It's beginning and end are defined by the interval borderlines, the apex is identified automatically. If the peak overlaps the neighboring peaks, their respective start and end points will be shifted and the peaks will be separated by vertical lines. New peaks cannot be created inside the interval of tangent skimming, nor close to the apex of an existing peak.

Note:

A two-step process must be used to create a peak in the area of a monotonous signal. First, the beginning (end) must be placed at the point where the apex ultimately should be placed and the end (or beginning) placed in its final position. Only then can the peak beginning or end be shifted by the Start (or End, as the case may be) command to the required position.

Add a positive peak

Invoking this command or using the Add Negative icon creates a new negative peak. It's beginning and end are defined by the interval borderlines, the apex is identified automatically. If the peak overlaps neighboring peaks, their respective start and end points will be shifted and the peaks will be separated by vertical lines. New peaks cannot be created inside the interval of tangent skimming, nor close to the apex of an existing peak.

Note:

A two-step process must be used to create a peak in the area of a monotonous signal. First, the beginning (end) must be placed at the point where the apex should ultimately be placed and the end (or beginning) placed at its final position. Only then can the peak beginning or end be shifted by the Start (or End, as the case may be) command to the required position.

Add a negative peak

Invoking this command or using the Force Peak Name icon lets the user to select a peak whose name should be changed and then opens the Force Peak Name dialog with a list of all peaks included in the calibration file. Alternatively, user can define its own name in the field on the top of the dialog.

Override peak name from calibration

 

Force Peak Name

Selected peak name will be set to the given peak.

Note:

A peak with the same name cannot appear twice in the Result Table - only the peak named so latest will hold it's name, other peaks with the same name will become uncalibrated.

Invoking this command or the Solvent Peak icon marks the selected peak as solvent peak; several peaks may be marked this way by repeatedly using the command. Solvent peak will be labeled by the letter S before the serial number (if displayed) in the Result Table and is excluded from the integration results.

Set a solvent peak

Invoking this command or the Group icon opens the Groups dialog used for the operations with peak groups.

Groups

ID

A group is marked by a single capital letter symbol (26 letters of the English alphabet can be used). If the identifier of a group already containing at least one peak is entered here, the group will be highlighted in the Existing Groups list.

Existing Groups

All defined groups containing at least one peak are listed here. When a calibrated calculation is used, the name taken from the calibration file is appended after the group identifier (provided the calibration file contains the relevant group). To transfer the group identified to the ID field, click the corresponding line.

Add

Adds peaks to the group selected in the ID field or creates a new group, if the one set in the ID field does not exist. All peaks with a maximum inside of the selected interval will be added to the group, it is not necessary to include the area to the peak start or end. A peak can only be included in a single group - only the last designation will thus apply.

Add peaks into a group

Delete

Deletes peaks form the group selected in the ID field. Only peaks assigned to that group and with maximum within the selected interval will be deleted.

Note:

If the Group ID field on the Graph Properties dialog is checked, the capital letter of a group that a peak belongs to will be indicated in the graph at the peak’s description.